Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins
نویسندگان
چکیده
We propose here the application of synaptic vesicle proteins isolated from rat brain as a sole substrate for the specific endoproteinase activities of all seven serotypes of Botulinum Neurotoxin (BoNT/A to G). In this study, we used these proteins for evaluating endopeptidase and receptor binding activity for detecting BoNT/A by western blot and surface plasmon resonance with 6.25 pM and 0.22 fM limit of detection, respectively. Substrate and receptor present in the synaptic vesicle proteins are very robust and stable for more than 6 months to use in BoNT detection. J o u r na l o f B iote rrorism&iodefe n s e ISSN: 2157-2526 Journal of Bioterrorism & Biodefense Citation: Singh MK, Chauhan V, Dhaked RK (2012) Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins. J Bioterr Biodef S2:008. doi:10.4172/2157-2526.S2-008
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